Journal: PLoS ONE

Article Title: Effects of Negative Pressure Wound Therapy on Mesenchymal Stem Cells Proliferation and Osteogenic Differentiation in a Fibrin Matrix

doi: 10.1371/journal.pone.0107339

Figure Lengend Snippet: Immediately before and 3, 7 days after incubation, cell clots were harvested and confocal microscopy was performed to detect fluorescent immunostaining for COL1. Results showed that the expression of COL1 was absent or weak in two groups before incubation, but stronger in NPWT group at day 3 and 7 after incubation. Bars = 50 µm.

Article Snippet: Then they were blocked with goat serum, and incubated with the specific rat primary antibodies against collagen type 1 (COL1) (1∶100, BOSTER, Wuhan, China) overnight at 4°C.

Techniques: Incubation, Confocal Microscopy, Immunostaining, Expressing

Journal: PLoS ONE

Article Title: Effects of Negative Pressure Wound Therapy on Mesenchymal Stem Cells Proliferation and Osteogenic Differentiation in a Fibrin Matrix

doi: 10.1371/journal.pone.0107339

Figure Lengend Snippet: NPWT-treated and static control group cell clots were harvested before and 1, 3, and 7 days after incubation (n = 3 per timepoint for each group) and analyzed by real-time RT-PCR. The expressions of the specific osteogenic genes (A) ALP, (B) OC, and (D) COL1 were higher in NPWT group than those in control group (* p <0.05, ** p <0.01). The NPWT group also expressed higher levels of the osteogenic transcription factor Cbfa1 (also known as Runx2) and the mechanosignaling molecule integrin β5 (ITGB5) (C) and (E) (* p <0.05, ** p <0.01).

Article Snippet: Then they were blocked with goat serum, and incubated with the specific rat primary antibodies against collagen type 1 (COL1) (1∶100, BOSTER, Wuhan, China) overnight at 4°C.

Techniques: Control, Incubation, Quantitative RT-PCR